Analysis Note
ControlBrain tissue
Application
Research CategoryNeuroscience
This Anti-Stargazin/Cacng2 Antibody, CT is validated for use in IC, IP, WB for the detection of Stargazin/Cacng2.
Immunoprecipitation:
2-5 µg of this antibody has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate.
Research Sub CategorySynapse & Synaptic Biology
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state.
There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin.
Epitope: C-terminus
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Format: Purified
Quality
Evaluated by western blot on RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).
Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected Stargazin in RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).
Specificity
Predicted to cross-react with mouse based on sequence similarity.
Recognizes Stargazin, MW ~36-39 kDa.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Target description
~36-39 kDa
This product has met the following criteria to qualify for the following awards: